一种催化胰岛素原转化为胰岛素的牛胰腺酶。
A bovine pancreatic enzyme catalyzing the conversion of proinsulin to insulin.
作者信息
Yip C C
出版信息
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1312-5. doi: 10.1073/pnas.68.6.1312.
Abstract
An enzyme that catalyzes the conversion of bovine proinsulin to insulin has been purified from a bovine pancreatic extract. The product of conversion was identified as insulin by aminoacid analysis and determination of carboxyl terminal aminoacid residues. The purified enzyme preparation showed one major protein band on polyacrylamide gel disc electrophoresis; it had a molecular weight of about 70,000 and an isoelectric point (pI) at a pH of 4.82.
摘要
一种催化牛胰岛素原转化为胰岛素的酶已从牛胰腺提取物中纯化出来。通过氨基酸分析和羧基末端氨基酸残基的测定，将转化产物鉴定为胰岛素。纯化的酶制剂在聚丙烯酰胺凝胶圆盘电泳上显示出一条主要蛋白带；其分子量约为70,000，等电点（pI）为pH 4.82。
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