Brinkley L L, Vickerman M M
J Embryol Exp Morphol. 1979 Dec;54:229-40.
Fetuses were obtained from CD-1 mice at a time estimated to be 12 h prior to vivo secondary palate closure. One of the palatal shelves of each partially dissected fetal head was lesioned in one of five ways, the other left intact to serve as control. Single transverse cuts extending the width of the shelf were made at one of three positions along the longitudinal axis of the shelf: one-third, one-half or two-thirds the shelf length estimated from the rostral edge. Some specimens were cut in two places, dividing the shelf into three equal segments. Another group received a lesion which separated the caudal third of the shelf from its maxillary connections. All specimens were cultured for 18 h. At the end of the culture period the heads were fixed, examined and the degree of elevation of each shelf piece assessed. Intact, control shelves of all preparations were elevated in the rostral two-thirds of the shelf, while the caudal third was partially elevated. Results seen in lesioned shelves depended upon both the size of the segment and the region of the shelf contained in the segment. The rostral two-thirds of the shelf, the presumptive hard palate, whether intact or in segments elevated without physical connections to neighboring shelf tissue. Thus, it is unlikely that this elevation requires a wave of contraction be transmitted from the caudal soft palate region. In contrast, the presumptive soft palate requires continuity with the rostral portions of the shelf both to maintain structural stability and to elevate.
在预计体内继发腭闭合前12小时从CD-1小鼠获取胎儿。将每个部分解剖的胎儿头部的一个腭突以五种方式之一进行损伤，另一个保持完整作为对照。在沿着腭突纵轴的三个位置之一进行延伸腭突宽度的单一横向切割：从吻端边缘估计的腭突长度的三分之一、二分之一或三分之二处。一些标本在两个位置进行切割，将腭突分成三个相等的部分。另一组接受的损伤是将腭突的尾端三分之一与其上颌连接分离。所有标本培养18小时。在培养期结束时，将头部固定、检查并评估每个腭突部分的抬高程度。所有标本完整的对照腭突在腭突的吻端三分之二处抬高，而尾端三分之一部分抬高。损伤腭突的结果取决于部分的大小以及该部分所含腭突的区域。腭突的吻端三分之二，即推定的硬腭，无论完整与否或分成几段，都能在没有与相邻腭突组织的物理连接的情况下抬高。因此，这种抬高不太可能需要从尾端软腭区域传递一波收缩。相比之下，推定的软腭需要与腭突的吻端部分保持连续性，以维持结构稳定性并实现抬高。
