Bulleit R F, Zimmerman E F
J Embryol Exp Morphol. 1985 Aug;88:265-79.
The intrinsic forces necessary for directing the reorientation of the secondary palate appear to reside in the anterior two thirds of the palate or presumptive hard palate. The hard palate could reorient regardless of whether it was intact or separated from the posterior third or presumptive soft palate. The soft palate could only reorient if the palate shelves are left intact. These intrinsic forces, within the hard palate, may be mediated by the mesenchymal cells, their extracellular matrix, or the epithelium surrounding the shelves. This latter possibly was tested by removing the epithelium, from either the presumptive oral or nasal surface followed by measurement of reorientation in vitro. Only after removal of the oral epithelium was a significant inhibition in reorientation observed. The treatment used to remove the epithelium, EDTA and scraping, was shown to remove 41% of the oral epithelium leaving the majority of the basement membrane intact. The observed inhibition of reorientation did not appear to be a consequence of wound healing. Creation of wounds twice the area that was observed after treatment with EDTA and scraping inhibited reorientation minimally. These results suggest that the epithelium and particularly the anterior oral epithelium plays a major role in the reorientation of the murine secondary palate.
引导次生腭重新定向所需的内在力量似乎存在于腭的前三分之二或假定的硬腭中。无论硬腭是完整的还是与后三分之一或假定的软腭分离，它都能重新定向。只有当腭板保持完整时，软腭才能重新定向。硬腭内的这些内在力量可能由间充质细胞、其细胞外基质或围绕腭板的上皮介导。通过从假定的口腔或鼻表面去除上皮，然后在体外测量重新定向，对后者进行了可能的测试。只有在去除口腔上皮后，才观察到重新定向受到显著抑制。用于去除上皮的处理方法（EDTA和刮除）显示可去除41%的口腔上皮，而大部分基底膜保持完整。观察到的重新定向抑制似乎不是伤口愈合的结果。制造面积为EDTA处理和刮除后观察到的两倍的伤口，对重新定向的抑制作用最小。这些结果表明，上皮，尤其是前口腔上皮在小鼠次生腭的重新定向中起主要作用。
