Johnson M H, Chakraborty J, Handyside A H, Willison K, Stern P
J Embryol Exp Morphol. 1979 Dec;54:241-61.
A rabbit antiserum to a mouse embryonal carcinoma cell line blocks compaction of cleaving mouse embryos. Cell division is not affected up to the 32-cell stage but intracellular junctions fail to develop. Removal of the antibody at this stage permits compaction to occur and a normal blastocyst develops. Prolonged decompaction beyond the 32-cell embryo results in an increasing proportion of malformed blastocysts in which trophectodermal cells predominate and functional inner cell mass (ICM) cells are reduced or absent. The relationship of compaction to the generation of ICM and trophectoderm lineages in the intact embryo is discussed.
一种针对小鼠胚胎癌细胞系的兔抗血清可阻止正在分裂的小鼠胚胎发生致密化。在32细胞阶段之前，细胞分裂不受影响，但细胞间连接无法形成。在此阶段去除抗体后，致密化过程得以发生，正常的囊胚得以发育。超过32细胞胚胎阶段后长时间的解致密化会导致畸形囊胚的比例增加，其中滋养外胚层细胞占主导，功能性内细胞团（ICM）细胞减少或缺失。本文讨论了致密化与完整胚胎中ICM和滋养外胚层谱系产生的关系。
