Vasseur M, Duprey P, Brûlet P, Jacob F
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1155-9. doi: 10.1073/pnas.82.4.1155.
The recombinant cDNA RecXVI, which hybridizes to the endo A mRNA, detects two specific bands on a Southern blot of EcoRI-digested genomic mouse DNA. We have screened a mouse genomic library with this cDNA and isolated these two sequences. Endo A is encoded by a 7.5-kilobase gene and by a 1.6-kilobase pseudogene devoid of introns. A repetitive sequence belonging to the B2 family is located in the third intron of the gene. We have observed that transcription of B2 sequences and of the endo A gene are mutually exclusive.
与内源性A mRNA杂交的重组cDNA RecXVI，在经EcoRI消化的小鼠基因组DNA的Southern印迹上检测到两条特异性条带。我们用该cDNA筛选了小鼠基因组文库并分离出这两个序列。内源性A由一个7.5千碱基的基因和一个没有内含子的1.6千碱基的假基因编码。属于B2家族的一个重复序列位于该基因的第三个内含子中。我们观察到B2序列和内源性A基因的转录是相互排斥的。
