压力诱导沉降核糖体的解离：对沉降模式的影响
Pressure-induced dissociation of sedimenting ribosomes: effect on sedimentation patterns.
作者信息
Infante A A, Baierlein R
出版信息
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1780-5. doi: 10.1073/pnas.68.8.1780.
Abstract
Anomalous sedimentation patterns arise when free ribosomes from sea urchin eggs are centrifuged at high speeds. Pressure-induced dissociation of the ribosomes during sedimentation can explain the peculiar behavior; the assumption of such dissociation also yields estimates of the equilibrium constant (as a function of KCl concentration) and the change in molecular volume (500 +/- 100 ml/mol) in the reaction: subunits right arrow over left arrow ribosome. Such dissociation during centrifugation may explain many experiments in which apparent reduced sedimentation coefficients for ribosomes, and increased coefficients for the subunits, have been ascribed to conformational changes.
摘要
当对海胆卵中的游离核糖体进行高速离心时，会出现异常的沉降模式。沉降过程中压力诱导的核糖体解离可以解释这种特殊行为；这种解离的假设还能得出平衡常数（作为氯化钾浓度的函数）以及反应中亚基⇌核糖体过程中分子体积变化（500±100毫升/摩尔）的估计值。离心过程中的这种解离可能解释了许多实验现象，在这些实验中，核糖体明显降低的沉降系数以及亚基增加的沉降系数被归因于构象变化。
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