Izzard S, Tedeschi H
Proc Natl Acad Sci U S A. 1970 Oct;67(2):702-9. doi: 10.1073/pnas.67.2.702.
An earlier study has indicated that the swelling of isolated mitochondria induced by inorganic phosphate (P(i)) can be accounted for largely or completely by the accumulation of ions. In the present study, a similar uptake was shown to be induced by a wider range of P(i) concentrations. Addition of ADP, KCN, or 2,4-dinitrophenol initiates a shrinkage which can be accounted for by the efflux of ions. The results are consistent with the explanations that (a) P(i) induces a transport of ions and a concomitant osmotic swelling, and (b) the addition of substances which compete or interfere with the energy available for transport results in ion efflux and a corresponding mitochondrial shrinkage. The results are not consistent with proposals that the changes in the light scattered by mitochondrial suspensions with alterations in metabolic states reflect a mechanochemical coupling phenomenon.
一项较早的研究表明，无机磷酸盐（P(i)）诱导的分离线粒体肿胀在很大程度上或完全可以由离子积累来解释。在本研究中，已表明在更宽范围的P(i)浓度下会诱导类似的摄取。添加ADP、KCN或2,4-二硝基苯酚会引发收缩，这可以由离子外流来解释。这些结果与以下解释一致：(a) P(i)诱导离子运输并伴随渗透性肿胀，以及(b) 添加竞争或干扰可用于运输的能量的物质会导致离子外流和相应的线粒体收缩。这些结果与线粒体悬浮液随代谢状态改变而散射光的变化反映机械化学偶联现象的提议不一致。
