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沙门氏菌O抗原的聚合机制：脂连接中间体的利用

Mechanism of polymerization of the Salmonella O-antigen: utilization of lipid-linked intermediates.

作者信息

Kanegasaki S, Wright A

出版信息

Proc Natl Acad Sci U S A. 1970 Oct;67(2):951-8. doi: 10.1073/pnas.67.2.951.

DOI:10.1073/pnas.67.2.951
PMID:5289031
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC283297/
Abstract

Cell envelope fractions from Salmonella can utilize exogenous lipid-linked intermediates for the synthesis of polymeric O-antigen. We describe a method for preparing aqueous suspensions of lipid intermediates and show that freezing and thawing of cell envelope-lipid intermediate mixtures is required for efficient synthesis. The lipid intermediates move freely in the hydrophobic environment of the membrane.

摘要

沙门氏菌的细胞膜组分可利用外源脂质连接中间体来合成聚合O抗原。我们描述了一种制备脂质中间体水悬浮液的方法，并表明细胞膜-脂质中间体混合物的冻融对于高效合成是必需的。脂质中间体在膜的疏水环境中自由移动。

相似文献

1
Mechanism of polymerization of the Salmonella O-antigen: utilization of lipid-linked intermediates.沙门氏菌O抗原的聚合机制：脂连接中间体的利用
Proc Natl Acad Sci U S A. 1970 Oct;67(2):951-8. doi: 10.1073/pnas.67.2.951.
2
Glucosylation of lipopolysaccharide in Salmonella: biosynthesis of O antigen factor 12 2 . I. Over-all reaction.沙门氏菌中脂多糖的糖基化：O抗原因子12 2的生物合成。I. 总体反应。
J Biol Chem. 1971 Jun 25;246(12):3902-11.
3
Glucosylation of lipopolysaccharide in Salmonella: biosynthesis nof O antigen factor n12 2 . II. Structure of the lipid intermediate.沙门氏菌中脂多糖的糖基化：O抗原因子n12 2的生物合成。II. 脂质中间体的结构。
J Biol Chem. 1971 Jun 25;246(12):3912-9.
4
Mutants of group D1 Salmonella carrying the somatic antigen of group A organisms: evidence for the lack of cytidine diphosphate paratose-2-epimerase activity.携带A群生物体体细胞抗原的D1群沙门氏菌突变体：缺乏胞苷二磷酸副糖-2-表异构酶活性的证据。
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J Biol Chem. 1972 Jun 25;247(12):3973-86.
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[Partial purification of glycosyltransferases participating in the biosynthesis of Salmonella anatum and S. kentucky O-antigens using high performance gel chromatography].[利用高效凝胶色谱法对参与鸭沙门氏菌和肯塔基沙门氏菌O抗原生物合成的糖基转移酶进行部分纯化]
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Studies of phospholipid-requiring bacterial enzymes. 3. Purification and properties of uridine diphosphate glucose:lipopolysaccharide glucosyltransferase I.对需要磷脂的细菌酶的研究。3. 尿苷二磷酸葡萄糖：脂多糖葡萄糖基转移酶I的纯化及性质
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Biosynthesis of enterobacterial common antigen requires dTDPglucose pyrophosphorylase determined by a Salmonella typhimurium rfb gene and a Salmonella montevideo rfe gene.肠杆菌共同抗原的生物合成需要由鼠伤寒沙门氏菌rfb基因和蒙得维的亚沙门氏菌rfe基因所决定的dTDP葡萄糖焦磷酸化酶。
J Bacteriol. 1986 Nov;168(2):715-21. doi: 10.1128/jb.168.2.715-721.1986.

引用本文的文献

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EcoSal Plus. 2023 Dec 12;11(1):eesp00202022. doi: 10.1128/ecosalplus.esp-0020-2022. Epub 2023 Jan 9.
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In vitro bacterial polysaccharide biosynthesis: defining the functions of Wzy and Wzz.体外细菌多糖生物合成：定义 Wzy 和 Wzz 的功能。
Nat Chem Biol. 2010 Jun;6(6):418-23. doi: 10.1038/nchembio.351. Epub 2010 Apr 25.
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Biosynthesis of peptidoglycan in Gaffkya homari: reactivation of membranes by freeze-thawing in the presence and absence of walls.龙虾加夫基菌中肽聚糖的生物合成：在有壁和无壁情况下通过冻融使细胞膜重新激活
J Bacteriol. 1982 May;150(2):535-44. doi: 10.1128/jb.150.2.535-544.1982.
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The role of lipid-linked activated sugars in glycosylation reactions.脂连接活化糖在糖基化反应中的作用。
J Bioenerg. 1973 Jan;4(1):239-51. doi: 10.1007/BF01516060.
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Osmotic-sensitive mutant of Salmonella typhimurium.鼠伤寒沙门氏菌的渗透敏感突变体。
J Bacteriol. 1972 Mar;109(3):1273-83. doi: 10.1128/jb.109.3.1273-1283.1972.
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Deacylation of bacterial lipopolysaccharide in rat hepatocytes in vitro.体外大鼠肝细胞中细菌脂多糖的脱酰基作用
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Localization of the terminal steps of O-antigen synthesis in Salmonella typhimurium.鼠伤寒沙门氏菌中O抗原合成终末步骤的定位
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Mutants of Salmonella anatum that block bacteriophage epsilon infection at early stages.鸭沙门氏菌的突变体，其在早期阶段阻断噬菌体ε感染。
J Bacteriol. 1976 Jul;127(1):7-13. doi: 10.1128/jb.127.1.7-13.1976.

本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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Studies on the chemical basis of the phage conversion of O-antigens in the E-group Salmonellae.E群沙门氏菌中O抗原噬菌体转化的化学基础研究。
Biochemistry. 1962 Mar;1:323-35. doi: 10.1021/bi00908a020.
3
SEPARATION OF SUGAR NUCLEOTIDES, PHOSPHORIC ESTERS AND FREE SUGARS BY PAPER CHROMATOGRAPHY WITH SOLVENTS CONTAINING BORATES OF ORGANIC BASES.用含有机碱硼酸盐的溶剂通过纸色谱法分离糖核苷酸、磷酸酯和游离糖
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4
BIOSYNTHESIS OF CELL WALL POLYSACCHARIDE IN MUTANT STRAINS OF SALMONELLA. IV. SYNTHESIS OF S-SPECIFIC SIDE-CHAIN.沙门氏菌突变株中细胞壁多糖的生物合成。IV. S-特异性侧链的合成。
Biochem Biophys Res Commun. 1965 Apr 23;19:322-7. doi: 10.1016/0006-291x(65)90462-6.
5
ENZYMATIC SYNTHESIS OF THE SALMONELLA O-ANTIGEN.沙门氏菌O抗原的酶促合成
Proc Natl Acad Sci U S A. 1964 Nov;52(5):1302-9. doi: 10.1073/pnas.52.5.1302.
6
A CHEMICAL BASIS FOR THE HOST-INDUCED MODIFICATION OF T-EVEN BACTERIOPHAGES.宿主诱导T偶数噬菌体修饰的化学基础
Proc Natl Acad Sci U S A. 1963 Aug;50(2):300-5. doi: 10.1073/pnas.50.2.300.
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The direction of chain growth in Salmonella anatum O-antigen biosynthesis.
Biochem Biophys Res Commun. 1967 Aug 7;28(3):334-9. doi: 10.1016/0006-291x(67)90314-2.
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Biosynthesis of bacterial lipopolysaccharide. V. Lipid-linked intermediates in the biosynthesis of the O-antigen groups of Salmonella typhimurium.细菌脂多糖的生物合成。V. 鼠伤寒沙门氏菌O抗原基团生物合成中的脂质连接中间体。
Proc Natl Acad Sci U S A. 1965 Jul;54(1):228-35. doi: 10.1073/pnas.54.1.228.
9
Structure of a lipid intermediate in cell wall peptidoglycan synthesis: a derivative of a C55 isoprenoid alcohol.细胞壁肽聚糖合成中脂质中间体的结构：一种C55类异戊二烯醇的衍生物。
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1878-84. doi: 10.1073/pnas.57.6.1878.
10
Evidence for an intermediate stage in the biosynthesis of the Salmonella O-antigen.沙门氏菌O抗原生物合成中间阶段的证据。
Proc Natl Acad Sci U S A. 1965 Jul;54(1):235-41. doi: 10.1073/pnas.54.1.235.