Koupparis M A, Walczak K M, Malmstadt H V
J Pharm Sci. 1979 Dec;68(12):1479-82. doi: 10.1002/jps.2600681204.
A reaction rate method for the determination of beta-methasone, betamethasone valerate, triamcinolone acetonide, and fluocinolone acetonide is described. The method is based on a modification of the widely accepted blue tetrazolium reaction. Analysis times of 30--70 sec are required. Relative standard deviations of 0.3--1.9% are obtained, and the analytical working curves are linear. Analysis of pharmaceutical skin preparations by the new method gave results that correlated well with the time-consuming standard equilibrium method. Analysis of betamethasone and betamethasone valerate mixtures by measuring absorbance values at two different times was performed also.
本文描述了一种用于测定倍他米松、戊酸倍他米松、曲安奈德和醋酸氟轻松的反应速率法。该方法基于对广泛认可的蓝四氮唑反应的改进。分析时间需要30 - 70秒。相对标准偏差为0.3 - 1.9%，分析工作曲线呈线性。用新方法对药用皮肤制剂进行分析，所得结果与耗时的标准平衡法相关性良好。还通过在两个不同时间测量吸光度值对倍他米松和戊酸倍他米松混合物进行了分析。
