Hart P D, Rees R J, Niven J S
Clin Exp Immunol. 1968 Jan;3(1):91-8.
Methotrexate administered over a period of 8 weeks reduced tuberculin skin sensitivity in guinea-pigs, the effect waning in the later part of the period. The delayed administration of folinic acid successfully reduced the toxicity of methotrexate so that the dose of the latter could be increased three-fold; there was then no impairment, and probably some increase, of its inhibition of tuberculin sensitivity. However, the suppression was not complete even with these large doses of the drug. In the spleen at the termination of an experiment, there was a striking diminution in the number of small lymphocytes in animals given methotrexate with or without folinic acid, compared with untreated animals. Animals given methotrexate plus folinic acid showed much larger spleens than did untreated animals, the spleen of the single survivor of the group on methotrexate alone being intermediate in size. There was a haemopoietic reaction corresponding in degree to the size of the spleen in the three groups.
在8周的时间内给予甲氨蝶呤可降低豚鼠的结核菌素皮肤敏感性，该效应在这段时间的后期逐渐减弱。亚叶酸钙的延迟给药成功降低了甲氨蝶呤的毒性，从而使后者的剂量可以增加三倍；此时其对结核菌素敏感性的抑制没有受损，甚至可能有所增强。然而，即使使用这些大剂量的药物，抑制也不完全。在实验结束时，与未治疗的动物相比，给予甲氨蝶呤（无论是否加用亚叶酸钙）的动物脾脏中小淋巴细胞的数量显著减少。给予甲氨蝶呤加亚叶酸钙的动物脾脏比未治疗的动物大得多，仅接受甲氨蝶呤治疗组的唯一存活者的脾脏大小介于两者之间。三组中均存在与脾脏大小程度相应的造血反应。
