Vannini G L, Fasulo M P, Dall'Olio G
Microbios. 1979;24(95):41-9.
The dermatophytic fungus Microsporum cookei was cultivated for 24 h in the presence of subinhibitory and inhibitory concentrations (50 and 100 micrograms/ml) of Phosfon D, a growth retardant for higher plants also affecting fungal development, and its toxic effects were examined at the ultrastructural level. In both treatments, Phosfon D attacked the membranes, whose structural integrity was clearly compromised with damage of particular severity to mitochondria, nuclei and endoplasmic reticulum. In the instance of fungal growth suppression, the compound also caused plasmolytic and autolytic phenomena, sometimes accompanied by plasma membrane breakages. The submicroscopic effects observed confirm that Prosfon D is an antifungal compound which displays its toxic effects in the area of lipid metabolism, probably preventing the synthesis of fundamental components of the cellular membranes, such as unsaturated fatty acids and sterols.
将皮肤癣菌小孢子菌在亚抑制浓度和抑制浓度（50和100微克/毫升）的福斯方D存在下培养24小时，福斯方D是一种对高等植物有生长抑制作用且也影响真菌发育的物质，并在超微结构水平上检查其毒性作用。在两种处理中，福斯方D都攻击了膜，其结构完整性明显受损，线粒体、细胞核和内质网受到特别严重的损伤。在抑制真菌生长的情况下，该化合物还引起质壁分离和自溶现象，有时伴有质膜破裂。观察到的亚微观效应证实，福斯方D是一种抗真菌化合物，其在脂质代谢领域发挥毒性作用，可能阻止细胞膜基本成分如不饱和脂肪酸和甾醇的合成。
