猪胃黏膜中组胺及其代谢产物的测定
Determination of histamine and its metabolic products in the pig gastric mucosa.
作者信息
Schippert B, Kovar K A, Sewing K F
出版信息
Pharmacology. 1979;19(2):86-95. doi: 10.1159/000137293.
This paper describes analytical techniques for quantitative and qualitative determination of histamine and its metabolites (N tau-methylhistamine, N alpha-methylhistamine and N alpha, N alpha-dimethylhistamine) in the pig gastric mucosa. These metabolites and N alpha-acetylhistamine, imidazolyl-4-acetic acid and N tau-methylimidazolylacetic acid were synthesized as reference compounds and analyzed by using the dansylation technique. TLC of dansylated mucosal extracts in various solvents (in situ fluorescence measurements) in combination with TLC/IR and TLC/MS transfer technique demonstrated the presence of histamine. The only metabolite was N tau-methylhistamine.
本文描述了用于定量和定性测定猪胃黏膜中组胺及其代谢产物（Nτ-甲基组胺、Nα-甲基组胺和Nα，Nα-二甲基组胺）的分析技术。这些代谢产物以及Nα-乙酰组胺、咪唑基-4-乙酸和Nτ-甲基咪唑基乙酸被合成为参考化合物，并采用丹磺酰化技术进行分析。在各种溶剂中对丹磺酰化的黏膜提取物进行薄层色谱分析（原位荧光测量），结合薄层色谱/红外光谱和薄层色谱/质谱转移技术，证实了组胺的存在。唯一的代谢产物是Nτ-甲基组胺。
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