Rickman L R, Robson J
Bull World Health Organ. 1970;42(6):911-6.
The authors describe a simple test (the blood incubation infectivity test) by which Trypanosoma brucei (sensu stricto) may be differentiated from T. rhodesiense without recourse to human volunteers. The method consists in incubating the strain of trypanosome under test for 5 hours at 37 degrees C in vitro in human blood, followed by observation of the effect of this procedure on the strain's infectivity to rats.Thirteen strains of T. rhodesiense were investigated; in each, the ability to infect rats was retained after incubation. In all 6 strains of man-tested T. brucei, it was destroyed.The consistency of the results with proven strains suggests very strongly that the blood incubation infectivity test provides a valid means of differentiating these parasites.
作者描述了一种简单的试验（血液培养感染性试验），通过该试验可以在不借助人类志愿者的情况下，将布氏锥虫（狭义）与罗德西亚锥虫区分开来。该方法包括将待测锥虫菌株在体外37℃的人血中培养5小时，然后观察此过程对该菌株感染大鼠能力的影响。对13株罗德西亚锥虫进行了研究；每株在培养后仍保留感染大鼠的能力。在所有6株经人体试验的布氏锥虫中，这种能力被破坏。这些结果与已证实的菌株的一致性非常有力地表明，血液培养感染性试验提供了一种区分这些寄生虫的有效方法。
