Woodruff M F, Inchley M P
Clin Exp Immunol. 1971 Dec;9(6):839-51.
The cytotoxic titre of rabbit-anti-mouse thymocyte IgG (ALG), determined by a modified test in which the cells were first incubated with ALG, then washed and re-incubated with guinea-pig C, ranged from 3110 to 5470. The cytolytic efficiency (i.e. the reciprocal of the mean number of IgG molecules attached per cell under conditions in which 50% lysis occurs in the presence of C), measured with I-ALG, was about 0·002%. When γ IgG from guinea-pigs immunized with rabbit IgG was added prior to the second incubation the cytotoxic titre was augmented up to ten-fold and the cytotoxic efficiency up to twenty-fold. The same γ preparation resulted in up to 300-fold augmentation in a haemolytic system. In the thymocyte-ALG system, unlike the haemolytic system, augmentation was maximal at reduced concentrations of γ. The mechanism of augmentation by γ has been investigated using I-ALG and I- γ IgG.
通过改良试验测定的兔抗小鼠胸腺细胞IgG（抗淋巴细胞球蛋白，ALG）的细胞毒性效价范围为3110至5470，该改良试验是先将细胞与ALG孵育，然后洗涤并与豚鼠补体（C）再次孵育。用碘标记的ALG（I-ALG）测得的细胞溶解效率（即在补体存在下发生50%溶解的条件下，每个细胞附着的IgG分子平均数的倒数）约为0.002%。在第二次孵育前加入用兔IgG免疫的豚鼠的γIgG后，细胞毒性效价增加到原来的十倍，细胞毒性效率增加到原来的二十倍。相同的γ制剂在溶血系统中可导致高达300倍的增加。在胸腺细胞-ALG系统中，与溶血系统不同，γ浓度降低时增强作用最大。已使用I-ALG和I-γIgG研究了γ增强作用的机制。
