[Features of RNA synthesis in isolated cell nuclei of M-1 sarcoma and rat liver].

As evidenced from investigation of patterns of RNA synthesis in cell nuclei of sarcoma M-1 and rat liver, RNA-polymerase activity depends on the presence of ribonucleoside triphosphates in the incubation medium. Inhibition of polymerase reaction was noted in the presence of DNAase, RNAase and actinomycin D. An increase in ionic strength of incubation medium induced a sharp increase in C14-UMP incorporation into RNA by cell nuclei of sarcoma M-1 and normal liver compared to regenerating liver and was followed by changes in nucleotide pattern of formed RNA with prevalence of AU-type As evidenced from investigation of products of RNA-polymerase reaction in sucrose density gradient, RNA synthesized by cell nuclei of sarcoma M-1 is represented by a low molecular weight fraction.