Perevoshchikova K A, Parinov V Ia, Vavilina G P, Solov'eva L Ia
Vopr Med Khim. 1971 Jul-Aug;17(4):439-43.
Three chemical methods for determination diethylstilbestrol (DES) in biological material (Jons and Deferage, 1953; Ponder, 1966; Official Method of Pharmacopoeia, USA, 1965) were compared. The most adequate method for determination of DES in animal tissue is the Official method of Pharmacopoeia based on a specific reaction. The method yields reproducible results. Average recovery of pure DES added to biological material was 78%. The method by Jons and Deferage though providing recovery of 86% of the added DES is based on nonspecific reaction with SbCl5, which resulted in too high results. Method of Pharmacopoeia (USA) was used to determine residual amounts of DES in tissues of a rabbit, implanted with 0.1 mg DES per 1 kg weight. One month after implantation, trace amounts of DES were detected only in muscle tissues. Two and three months after implantation, the residual amounts of DES in the rabbit tissues were not detected.
比较了三种测定生物材料中己烯雌酚（DES）的化学方法（Jons和Deferage，1953年；Ponder，1966年；美国药典官方方法，1965年）。测定动物组织中DES的最合适方法是基于特定反应的药典官方方法。该方法可产生可重复的结果。添加到生物材料中的纯DES的平均回收率为78%。Jons和Deferage的方法虽然添加的DES回收率为86%，但该方法基于与SbCl5的非特异性反应，导致结果过高。美国药典方法用于测定每1千克体重植入0.1毫克DES的兔子组织中DES的残留量。植入后一个月，仅在肌肉组织中检测到痕量的DES。植入后两个月和三个月，未检测到兔子组织中DES的残留量。
