Watanabe T, Hirayama T, Takahashi T, Kokubo T, Ikeda M
Toxicology. 1979 Sep;14(1):1-22. doi: 10.1016/0300-483x(79)90088-x.
Young male Wistar rats were fed with chows containing marketed Hizikia preparation. Hizikia-arsenic could be absorbed at the rate comparable to arsenic trioxide, and accumulated primarily in blood. Intake and excretion of arsenic from Hizikia-diet reached a steady state within 4 days of administration as was the case of arsenic trioxide. Biological half-life in blood and urine was also essentially the same in the 2 groups. Rats kept for 3 weeks on the chows containing up to 20% Hizikia did not show significant growth retardation as compared with the controls, while those on 50% Hizikia-diet were markedly emaciated. In a 38-week feeding experiment, 4 groups of rats were given 20% Hizikia-diets made from various Hizikia preparations (final arsenic concentrations; 9, 16, 21 and 21 ppm, respectively). For positive controls, 2 groups were given arsenic trioxide diets (24 and 50 ppm as arsenic). Growth retardation was minimum both in Hizikia and arsenic groups with no relation to the arsenic dose. Changes in hematology, blood biochemistry and pathology of major organs and skin were also essentially negative in all groups. The maximum no-effect level observed in the present study was compared with the possible amount of arsenic taken via seaweed in mankind.
将年轻雄性Wistar大鼠喂以含有市售羊栖菜制剂的食物。羊栖菜-砷的吸收率与三氧化二砷相当，且主要在血液中蓄积。羊栖菜饮食中砷的摄入和排泄在给药4天内达到稳态，三氧化二砷的情况也是如此。两组血液和尿液中的生物半衰期也基本相同。与对照组相比，在含有高达20%羊栖菜的食物上饲养3周的大鼠未表现出明显的生长迟缓，而食用50%羊栖菜饮食的大鼠则明显消瘦。在一项为期38周的喂养实验中，4组大鼠被给予由各种羊栖菜制剂制成的20%羊栖菜饮食（最终砷浓度分别为9、16、21和21 ppm）。作为阳性对照，2组大鼠被给予三氧化二砷饮食（砷含量为24和50 ppm）。羊栖菜组和砷组的生长迟缓均最小，且与砷剂量无关。所有组的血液学、血液生化以及主要器官和皮肤的病理学变化也基本呈阴性。将本研究中观察到的最大无作用水平与人类通过海藻摄入的可能砷量进行了比较。
