Schleich T, Goldstein J
Science. 1965 Nov 26;150(3700):1168-70. doi: 10.1126/science.150.3700.1168.
When S(35)-labeled soluble RNA from Escherichia coli K 38 is subjected to gel filtration, four fractions of RNA are obtained by elution. Only one RNA fraction, the transfer RNA, contains sulfur, presumably as thionucleotides. Treatment with ribonuclease suggests that the incorporated sulfur is an integral part of the polynucleotide chain; digestion with alkali yields a mixture of products containing sulfur, the major one being eluted in a position similar to uridine diphosphate upon Dowex-l chromatography. Analysis by countercurrent distribution of S(35)-labeledtransfer RNA from E. coli B reveals that the incorporated sulfur is found in many RNA's that accept amino acids, but the possibility remains that not all acceptor RNA's contain sulfur.
当用凝胶过滤法处理来自大肠杆菌K 38的S(35)标记的可溶性RNA时，通过洗脱可得到四个RNA组分。只有一个RNA组分，即转移RNA，含有硫，推测是以硫代核苷酸的形式存在。用核糖核酸酶处理表明，掺入的硫是多核苷酸链的一个组成部分；用碱消化产生含硫产物的混合物，主要产物在Dowex-1柱层析上的洗脱位置与尿苷二磷酸相似。对来自大肠杆菌B的S(35)标记的转移RNA进行逆流分配分析表明，掺入的硫存在于许多接受氨基酸的RNA中，但仍有可能并非所有的受体RNA都含硫。
