Vogt R, Mehnert W H, Schmidt H E, Altenbrunn H J
Z Exp Chir. 1979 Aug;12(4):209-15.
The authors report about investigations for localization of tumours in vivo by scintigrafic iddntification of Clostridium butyricum Jena H8 by 131I-labelled antibodies and by 131I-labelled F(ab')2-fragment antibodies in mouse transplantation-tumour UVT 15264. The application of 131I-labelled anti-clostridial-rod-antibodies in tumour bearing mice demonstrates increase of radioactivity in tumours of animals, pretreated by spores, as well as in animals without pretreatment. These appearently unspecific increases of radioactivity could not be demonstrated by application of 131I-labelled F(ab')2-fragment antibodies directed against clostridial rods, but also the specific fixation of 131I-labelled F(ab')2-fragment antibodies in tumours of mice, pretreated by clostridial spores, could not be demonstrated. The results are discussed with regard to further investigations.
作者报告了通过用¹³¹I标记的抗体和¹³¹I标记的F(ab')₂片段抗体对丁酸梭菌耶拿H8进行闪烁显像来在体内定位小鼠移植瘤UVT 15264中肿瘤的研究。在荷瘤小鼠中应用¹³¹I标记的抗梭菌杆抗体表明，经孢子预处理的动物肿瘤以及未预处理的动物肿瘤中的放射性均增加。应用针对梭菌杆的¹³¹I标记的F(ab')₂片段抗体无法证明这些明显非特异性的放射性增加，但也无法证明¹³¹I标记的F(ab')₂片段抗体在经梭菌孢子预处理的小鼠肿瘤中的特异性结合。针对进一步的研究对结果进行了讨论。
