Schor S L, Johnson R T, Mullinger A M
J Cell Sci. 1975 Nov;19(2):281-303. doi: 10.1242/jcs.19.2.281.
A method is described for the isolation, according to size, of mini segregants produced by the abnormal cleavage of reversibly arrested mitotic HeLa cells. Many of these mini segregants contain small amounts of DNA, as judged by Feulgen staining and chromosome analysis. After fusion with mitotic HeLa cells, the interphase chromosomes of the mini segregants are seen as either monovalent or bivalent prematurely condensed chromosomes (PCC), some of which are damaged. A proportion of isolated mini segregants synthesize DNA, RNA and protein. Fusion of mini segregants with interphase HeLa cells gives rise to cells with 'hybrid' karyotypes.
本文描述了一种根据大小分离由可逆性停滞的有丝分裂海拉细胞异常分裂产生的微小分离体的方法。通过福尔根染色和染色体分析判断，许多这些微小分离体含有少量DNA。与有丝分裂海拉细胞融合后，微小分离体的间期染色体表现为单价或二价的早熟凝集染色体（PCC），其中一些受损。一部分分离出的微小分离体可合成DNA、RNA和蛋白质。微小分离体与间期海拉细胞融合会产生具有“杂交”核型的细胞。
