选择蔗糖依赖性大肠杆菌以获得包膜突变体和脆弱培养物。

Selection of sucrose-dependent Escherichia coli to obtain envelope mutants and fragile cultures.

作者信息

Mangiarotti G, Apirion D, Schlessinger D

出版信息

Science. 1966 Aug 19;153(3738):892-4. doi: 10.1126/science.153.3738.892.

DOI:10.1126/science.153.3738.892

Abstract

Mutants isolated as sucrose-dependent include many with apparent envelope defects, which fact often leads to filamentous growth or lysis in the absence of sucrose. One mutant can grow exponentially in 4 percent sucrose, but is very fragile: it releases all of its RNA and soluble protein when treated with 0.5 percent sodium deoxycholate. These characteristics permit the study of unstable structures and rapid processes in actively growing cells.

摘要

分离得到的蔗糖依赖性突变体包括许多明显存在包膜缺陷的突变体，这一事实常常导致在没有蔗糖的情况下出现丝状生长或裂解。一种突变体可以在4%的蔗糖中呈指数生长，但非常脆弱：用0.5%的脱氧胆酸钠处理时，它会释放出所有的RNA和可溶性蛋白质。这些特性有助于研究活跃生长细胞中的不稳定结构和快速过程。