Pfützner H, Schimmel D
Arch Exp Veterinarmed. 1979;33(3):429-37.
Thirty-two isolates of altered milk samples taken from cows with mastitis on three industrialised dairy cattle units were biochemically tested following five passes through no-inhibitor media, assessment of filtratability through 450-nm-membrane filter, and clone assay. Something between five and ten clones of each of the strains involved were tested for their growth properties both at 22 degrees C and in no-serum media, sensitivity to digitonin and capability of aesculin hydrolysis. TTC reduction (triphenyltetrazolium-chloride), as well as for decomposition of glucose, arginine, and urea. The properties found were characteristic of M. bovis, A. laidlawii, and A. axanthum, and of the family of mycoplasmataceaea in the strains of one of the stocks.
从三个工业化奶牛场患乳腺炎的奶牛采集的32份变质乳样分离株,在通过无抑制剂培养基传代五次、评估通过450纳米膜过滤器的过滤性以及克隆试验后,进行了生化测试。对各相关菌株的5到10个克隆在22摄氏度和无血清培养基中测试其生长特性、对洋地黄皂苷的敏感性以及七叶苷水解能力。测试了氯化三苯基四氮唑(TTC)还原以及葡萄糖、精氨酸和尿素的分解情况。所发现的特性是牛支原体、莱氏无胆甾原体和黄色无胆甾原体以及其中一批菌株中的支原体科的特征。