Schuh V, Sír J, Galliová J, Svandová E
Bull World Health Organ. 1966;35(4):557-61.
A comparison of the weight and photometric methods of primary assay of BCG vaccine has been made, using a vaccine prepared in albumin-free medium but containing Tween 80. In the weight method, the bacteria were trapped on a membrane filter; for photometry a Pulfrich Elpho photometer and an instrument of Czech origin were used. The photometric results were the more precise, provided that the measurements were made within two days of completion of growth; after this time the optical density of the suspension began to decrease slowly. The lack of precision of the weighing method is probably due to the small weight of culture deposit (which was almost on the limit of accuracy of the analytical balance) and to difficulties in the manipulation of the ultrafilter.
对不含白蛋白但含有吐温80的培养基中制备的卡介苗进行了重量法和光度法两种主要测定方法的比较。在重量法中，细菌被截留在膜滤器上；光度法使用普尔弗里希光电比色计和一台原产于捷克的仪器。如果在生长完成后的两天内进行测量，光度法的结果会更精确；在此之后，悬浮液的光密度开始缓慢下降。重量法缺乏精确性可能是由于培养物沉淀物重量较小（几乎处于分析天平的精度极限）以及超滤器操作困难所致。
