胰岛素生物合成的研究。鮟鱇胰岛组织孵育过程中亮氨酸-H3放射性的亚细胞分布。
Studies on insulin biosynthesis. Subcellular distribution of leucine-H3 radioactivity during incubation of goosefish islet tissue.
作者信息
Bauer G E, Lindall A W, Dixit P K, Lester G, Lazarow A
出版信息
J Cell Biol. 1966 Mar;28(3):413-21. doi: 10.1083/jcb.28.3.413.
Abstract
Islet tissue from the goosefish (Lophius piscatorius) was incubated in the presence of leucine-H(3). The tissue was then separated into subcellular fractions and the radioactivity determined in total acid alcohol-soluble proteins, insoluble proteins, and insulin. At any time, microsomal protein had a higher activity than secretion granule protein. Pulse-chase experiments further suggest the microsomes as primary sites of protein synthesis. The data are evidence for microsomal synthesis of insulin and for its subsequent transfer into the secretion granules.
摘要
将安康鱼（Lophius piscatorius）的胰岛组织在亮氨酸-H(3)存在的情况下进行孵育。然后将该组织分离成亚细胞级分，并测定总酸醇溶性蛋白、不溶性蛋白和胰岛素中的放射性。在任何时候，微粒体蛋白的活性都高于分泌颗粒蛋白。脉冲追踪实验进一步表明微粒体是蛋白质合成的主要场所。这些数据证明了胰岛素在微粒体中的合成以及随后向分泌颗粒的转移。
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