Rishi S, Rishi K K
Cytobios. 1979;24(93):33-42.
Mitotic and meiotic chromosomes of Trabala vishnu Lef. (Lasiocampidae, Lepidoptera) were studied after treatment with different concentrations of colchicine. Although with milder concentrations of the drug the chromosomes bore typical spherical dot-like or elongated forms, the clear monocentric nature of the chromosomes was revealed at the mitotic metaphase, and metaphase II by the use of stronger concentrations of the drug. It was possible to make karyograms of the mitotic and metaphase II chromosomes on the basis of the centromeric position. The fifty-two elements were grouped into six pairs of metacentric, seven pairs of sub-metacentric, eleven pairs of acrocentric, and two pairs of microchromosomes. Diakinetic figures in the colchicinized insects showed discrete chiasmata in the bivalents.
用不同浓度的秋水仙碱处理后，对绿尾大蚕蛾（枯叶蛾科，鳞翅目）的有丝分裂和减数分裂染色体进行了研究。尽管使用较低浓度药物时染色体呈现典型的球形点状或细长形，但在有丝分裂中期和减数第二次分裂中期，使用较高浓度药物时，染色体明显呈现单着丝粒性质。根据着丝粒位置，有可能绘制有丝分裂和减数第二次分裂染色体的核型图。52条染色体被分为6对中着丝粒染色体、7对亚中着丝粒染色体、11对近端着丝粒染色体和2对微染色体。经秋水仙碱处理的昆虫的终变期图像显示二价体中有离散的交叉。
