The mechanism of rosette formation between sheep red blood cells and L-A9 fibroblasts.

More than 20% of mouse L-A9 fibroblasts from rosettes with sheep red blood cells. This relatively weak interaction is mediated by receptors of glycoprotein nature; species-specific antigens of SRBC are part of, or lie in close proximity to, this binding. SRBC receptors are present on most L-A9 fibroblasts, neither Con A receptors nor MHL products are part of the SRBC receptors. However, Con A- and H-2 antibody-induced alterations in the membrane and cytoskeletal system accompanying cap formation produce a strong inhibition of rosette formation. This inhibition may be due to an interference into the adhesion by involving the cytoskeletal system in the redistribution processes because the same effect was obtained with Colcemid and cytochalasin B.