Chan J T, Spike T E, Trowbridge S T, Schroepfer G J
J Lipid Res. 1979 Nov;20(8):1007-19.
[3 alpha-3H]14 alpha-Methyl-5 alpha-cholest-7-en-3 beta-ol has been prepared by chemical synthesis. The metabolism of this compound has been studied in the 10,000 g supernatant fraction of liver homogenates of female rats. Efficient conversion to cholesterol was observed. Other labeled compounds recovered after incubation of [3 alpha-3H]14 alpha-methyl-5 alpha-cholest-7-en-3 beta-ol with the enzyme preparations include the unreacted substrate, 5 alpha-cholesta-7,14-dien-3 beta-ol, 5 alpha-cholesta-8,14-dien-3 beta-ol, cholesta-5,7-dien-3 beta-ol, 5 alpha-cholest-8(14)-en-3 beta-ol, 5 alpha-cholest-8-en-3 beta-ol, and 5 alpha-cholest-7-en-3 beta-ol. In addition, significant amounts of incubated radioactivity were recovered in steryl esters. The steroidal components of these esters were found to contain labeled 14 alpha-methyl-5 alpha-cholest-7-en-3 beta-ol, 5 alpha-cholesta-8,14-dien-3 beta-ol, 5 alpha-cholesta-7,14-dien-3 beta-ol, 5 alpha-cholest-8-en-3 beta-ol, 5 alpha-cholest-7-en-3 beta-ol, and cholesterol.
[3α-³H]14α-甲基-5α-胆甾-7-烯-3β-醇已通过化学合成制备。该化合物在雌性大鼠肝脏匀浆10000g上清液部分中的代谢情况已得到研究。观察到其可高效转化为胆固醇。[3α-³H]14α-甲基-5α-胆甾-7-烯-3β-醇与酶制剂孵育后回收的其他标记化合物包括未反应的底物、5α-胆甾-7,14-二烯-3β-醇、5α-胆甾-8,14-二烯-3β-醇、胆甾-5,7-二烯-3β-醇、5α-胆甾-8(14)-烯-3β-醇、5α-胆甾-8-烯-3β-醇和5α-胆甾-7-烯-3β-醇。此外，在甾醇酯中回收了大量孵育后的放射性物质。发现这些酯的甾族成分含有标记的14α-甲基-5α-胆甾-7-烯-3β-醇、5α-胆甾-8,14-二烯-3β-醇、5α-胆甾-7,14-二烯-3β-醇、5α-胆甾-8-烯-3β-醇、5α-胆甾-7-烯-3β-醇和胆固醇。
