Koenig E
J Cell Biol. 1967 Jul;34(1):265-74. doi: 10.1083/jcb.34.1.265.
RNA was analyzed in the whole rod cell and in the rod cell nucleus of the rabbit retina. The analysis was performed on rod cells or rod cell nuclei after they were isolated by microdissection and collected. The nuclei were denuded by selectively lysing inner and outer segments chemically. The rod cell contained an average of 0.65 micromicrog RNA. About 60% of the total RNA was nuclear. RNA concentration was of the order of 0.4% w/v. RNA base composition was determined for the whole rod cell and for the rod cell nucleus, and from it the base composition of cytoplasmic RNA was derived also. Microdissection of the retina revealed that each Müller cell had firmly attached to it a certain complement of visual cells, forming a bouquet-like arrangement. The unusual susceptibility of the inner and outer segments to lysis was regarded as an indication of an unique composition of the plasma membrane.
对兔视网膜的整个视杆细胞及其细胞核中的RNA进行了分析。分析是在通过显微切割分离并收集的视杆细胞或视杆细胞核上进行的。通过化学方法选择性地裂解内段和外段来去除细胞核。视杆细胞平均含有0.65微微克RNA。总RNA中约60%存在于细胞核中。RNA浓度约为0.4%（w/v）。测定了整个视杆细胞和视杆细胞核的RNA碱基组成，并由此得出细胞质RNA的碱基组成。视网膜的显微切割显示，每个穆勒细胞都牢固地附着有一定数量的视觉细胞，形成花束状排列。内段和外段对裂解异常敏感被认为是质膜独特组成的一个指标。
