雌性在大肠杆菌细菌接合中的作用。
Role for the female in bacterial conjugation in Escherichia coli.
作者信息
Freifelder D
出版信息
J Bacteriol. 1967 Aug;94(2):396-402. doi: 10.1128/jb.94.2.396-402.1967.
Abstract
Hfr and F' Lac male strains of Escherichia coli were mated with purine-requiring females which had been starved for purine. These females formed mating pairs with the males. However, a mating in the absence of purine markedly reduced the yield of recombinants. Transfer of F' Lac or of lambda prophage also occurred infrequently. It was concluded that deoxyribonucleic acid transfer from male to female requires some, as yet unknown, function of the female.
摘要
将大肠杆菌的高频重组（Hfr）和F' Lac雄性菌株与需要嘌呤且已处于嘌呤饥饿状态的雌性菌株进行交配。这些雌性菌株与雄性菌株形成了交配配对。然而，在缺乏嘌呤的情况下进行交配会显著降低重组体的产量。F' Lac或λ原噬菌体的转移也很少发生。得出的结论是，从雄性到雌性的脱氧核糖核酸转移需要雌性的某种目前尚不清楚的功能。
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