Swann D A
Biochem J. 1969 Oct;114(4):819-25. doi: 10.1042/bj1140819.
Physical and chemical methods were used to characterize hyaluronic acid before (fraction HAIIBI) and after (fraction HA-AA) treatment with ascorbic acid. Fraction HA-AA was recovered with an almost quantitative yield and was shown to be chemically identical with fraction HAIIBI by all the methods used. These two materials, however, differed markedly in their molecular sizes and degree of polydispersity. By using sedimentation, diffusion and sedimentation-equilibrium analyses, weight-average molecular weights of about 1.2x10(6) and 6.5x10(4) respectively were obtained for fractions HAIIBI and HA-AA. It is concluded from these results that hyaluronic acid has a molecular weight of about 65000 and that the polysaccharide chain of this molecule is not depolymerized by ascorbic acid. It is further proposed that hyaluronic acid molecules in the matrix of connective tissues are present either in an aggregated form or as subunits of heterogeneous macromolecules, and that it is the linkages responsible for the organization of these structures which are broken by ascorbic acid.
采用物理和化学方法对透明质酸在抗坏血酸处理之前（HAIIBI级分）和之后（HA - AA级分）进行表征。HA - AA级分的回收率几乎达到定量，并且通过所有使用的方法表明其与HAIIBI级分在化学性质上相同。然而，这两种物质在分子大小和多分散程度上有显著差异。通过沉降、扩散和沉降平衡分析，分别得到HAIIBI级分和HA - AA级分的重均分子量约为1.2×10⁶和6.5×10⁴。从这些结果可以得出结论，透明质酸的分子量约为65000，并且该分子的多糖链不会被抗坏血酸解聚。进一步推测，结缔组织基质中的透明质酸分子要么以聚集形式存在，要么作为异质大分子的亚基存在，并且是负责这些结构组织的连接被抗坏血酸破坏。
