从未经刺激和经植物血凝素激活的淋巴细胞中分离出的信使核糖核酸的翻译活性。
Translational activity of messenger ribonucleic acid isolated from unstimulated and phytohaemagglutinin-activated lymphocytes.
作者信息
Wallace D M, Jagus R, Benzie C R, Kay J E
出版信息
Biochem J. 1979 Nov 15;184(2):277-82. doi: 10.1042/bj1840277.
Abstract
Purified cytoplasmic poly(A)+ RNA isolated from unstimulated pig lymphocytes has the same ability to direct translation in a range of cell-free systems as the corresponding mRNA from 20h phytohaemagglutinin-activated lymphocytes. Additional methylation of the mRNA is not required for maximum protein synthesis in the wheat-germ cell-free system. Misleading results are obtained if the mRNA preparations used are not adequately purified, and a method suitable for routine assessment of the degree of purification achieved is described. Cell-free protein-synthesizing systems from unstimulated lymphocytes translate added lymphocyte mRNA with lower efficiency than do comparable systems from phytohaemagglutinin-activated lymphocytes, whatever the source of the mRNA used.
摘要
从未经刺激的猪淋巴细胞中分离得到的纯化细胞质多聚腺苷酸（poly(A)+）RNA，在一系列无细胞系统中指导翻译的能力，与来自经20小时植物血凝素激活的淋巴细胞的相应mRNA相同。在小麦胚芽无细胞系统中，mRNA的额外甲基化并非最大程度蛋白质合成所必需。如果所用的mRNA制剂未得到充分纯化，就会得到误导性结果，本文描述了一种适用于常规评估纯化程度的方法。无论所用mRNA的来源如何，来自未经刺激淋巴细胞的无细胞蛋白质合成系统翻译添加的淋巴细胞mRNA的效率，都低于来自植物血凝素激活淋巴细胞的可比系统。
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