Shefer S, Hauser S, Bekersky I, Mosbach E H
J Lipid Res. 1969 Nov;10(6):646-55.
The hepatic biosynthesis of bile salts in the rat has been shown to be controlled homeostatically by the quantity of bile salt returning to the liver via the portal circulation. The feedback mechanism was demonstrated in two kinds of experiments. In the first, rats with bile fistulas were infused intraduodenally with sodium taurocholate 12 hr after surgery. If the rate of infusion was greater than 10 mg per 100 g rat per hr, the increase in bile acid output normally observed in bile fistula rats was prevented. In the second type of experiment, the rats were infused with taurocholate 48-72 hr after biliary diversion, when bile acid output had reached a maximal value. Provided the rate of infusion exceeded 10 mg per 100 g rat per hr, bile acid secretion returned to the low levels observed in intact rats. Previous attempts to demonstrate the feedback control have been unsuccessful because too little bile salt was infused. The taurocholate pool of the experimental animals was measured as approximately 15 mg per 100 g rat; it was calculated from this and the above results that this pool circulated 10-13 times daily.
大鼠体内胆汁盐的肝脏生物合成已被证明通过经门静脉循环返回肝脏的胆汁盐量进行稳态控制。在两类实验中证实了这种反馈机制。在第一个实验中，术后12小时经十二指肠向患有胆瘘的大鼠输注牛磺胆酸钠。如果输注速率大于每100克大鼠每小时10毫克，通常在胆瘘大鼠中观察到的胆汁酸输出增加就会受到抑制。在第二类实验中，在胆汁改道48 - 72小时后向大鼠输注牛磺胆酸盐，此时胆汁酸输出已达到最大值。只要输注速率超过每100克大鼠每小时10毫克，胆汁酸分泌就会恢复到完整大鼠中观察到的低水平。以前试图证明这种反馈控制的尝试均未成功，因为输注的胆汁盐太少。实验动物的牛磺胆酸盐池经测量约为每100克大鼠15毫克；据此以及上述结果计算得出，这个池每天循环10 - 13次。
