Kapica L, Shaw C E
Can Med Assoc J. 1969 Nov 15;101(10):47-50.
A new differential colour medium prepared with an extract of potatoes and carrots produced ochre-brown pigmentation in Cryptococcus neoformans colonies, identical to that obtained with Guizotia abyssinica seed extract. The pigment formation was observed to be specific for this pathogenic yeast in a survey of 534 yeast-positive specimens during routine diagnostic work; among 47 C. neoformans-positive specimens were 27 sputa and bronchial aspirations, many overgrown by common yeasts.Detection of C. neoformans in the contaminated specimens was credited largely to the differential medium used. Twelve cases of cryptococcosis were investigated between 1962 and 1968, five of which presented involvement of the respiratory tract only.
一种用土豆和胡萝卜提取物制备的新型鉴别性培养基,可使新生隐球菌菌落产生赭褐色色素沉着,与用埃塞俄比亚画眉草种子提取物获得的色素沉着相同。在常规诊断工作对534份酵母阳性标本的调查中,观察到色素形成是这种致病酵母所特有的;在47份新生隐球菌阳性标本中,有27份是痰液和支气管吸出物,许多被常见酵母过度生长。污染标本中新生隐球菌的检测很大程度上归功于所使用的鉴别性培养基。1962年至1968年间对12例隐球菌病进行了调查,其中5例仅表现为呼吸道受累。