用于估计抗牛痘抗体的反向被动血凝抑制法。

Reversed passive haemagglutination-inhibition method for the estimation of antivaccinia antibodies.

作者信息

Nyerges G

出版信息

Immunology. 1969 Sep;17(3):333-7.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1455891/

Abstract

A reversed passive haemagglutination-inhibition (HAI) method has been evolved for measuring antivaccinia antibodies. Tanned sheep erythrocytes were sensitized with a bovine hyperimmune antivaccinia γ-globulin solution. The sensitized erythrocytes were agglutinated by a purified suspension of vaccinia virus. Haemagglutination was inhibited by sera containing antivaccinia antibodies and so the antivaccinia titres could be measured. Reversed passive HAI titres were compared with Hirst-type HAI, complement-fixing and neutralization titres in sera of persons vaccinated for the first time and also in persons revaccinated. A correlation was found between the reversed passive HAI titres and the neutralization titres.

摘要

已开发出一种反向被动血凝抑制（HAI）方法来检测抗牛痘抗体。用牛超免疫抗牛痘γ球蛋白溶液致敏鞣酸处理的绵羊红细胞。致敏红细胞被纯化的牛痘病毒悬液凝集。含抗牛痘抗体的血清可抑制血凝，因此可检测抗牛痘滴度。将首次接种疫苗者和再次接种疫苗者血清中的反向被动HAI滴度与赫斯特型HAI、补体结合和中和滴度进行比较。发现反向被动HAI滴度与中和滴度之间存在相关性。

相似文献

1

Reversed passive haemagglutination-inhibition method for the estimation of antivaccinia antibodies.用于估计抗牛痘抗体的反向被动血凝抑制法。

Immunology. 1969 Sep;17(3):333-7.

2

[Relation between antibody formation and immunity against cutaneous vaccinia virus infection in hens].[母鸡体内抗体形成与抗皮肤痘苗病毒感染免疫力之间的关系]

Zentralbl Bakteriol Orig. 1968;207(3):419-21.

3

[Immunological response of the rabbit to experimental infection with vaccinia virus].[兔对痘苗病毒实验性感染的免疫反应]

Ann Sclavo. 1971 Sep-Oct;13(5):648-56.

4

Determination of the sensitivity of the plaque virus neutralization test: comparison with the sensitivity of antibody detection in haemagglutination and complement fixation systems.噬斑病毒中和试验敏感性的测定：与血凝和补体结合系统中抗体检测敏感性的比较。

Acta Virol. 1969 Sep;13(5):457.

5

[Passive hemagglutination test used as method for detection of antibodies in vaccinia].[用作检测牛痘抗体方法的被动血凝试验]

Arch Roum Pathol Exp Microbiol. 1965 Sep;24(3):771-8.

6

Method for detecting influenza virus sensibilizing antigen by the antibody neutalization test.

Acta Virol. 1968 Mar;12(2):167-70.

7

Inactivated smallpox vaccine: immunogenicity of inactivated intracellular and extracellular vaccinia virus.灭活天花疫苗：灭活的细胞内和细胞外痘苗病毒的免疫原性。

J Gen Virol. 1971 Oct;13(1):19-25. doi: 10.1099/0022-1317-13-1-19.

8

[Effect of a cofactor on the sensitivity of the vaccinia virus neutralization reaction in tissue culture].

Lab Delo. 1971;12:733-6.

9

The immunogenicity of heat-inactivated vaccinia virus in rabbits.

J Hyg (Lond). 1968 Mar;66(1):89-107. doi: 10.1017/s0022172400040973.

10

[Humoral immune response of rabbits inoculated with bovine herpes virus type 1. Comparison of serum neutralization, complement fixation, immunodiffusion and passive hemagglutination tests].

Zentralbl Veterinarmed B. 1983 Apr;30(3):180-8.

引用本文的文献

1

Vaccinia virus: kinetics of the hemagglutination-inhibition test and preparation of hemagglutinin.牛痘病毒：血凝抑制试验的动力学及血凝素的制备

Arch Gesamte Virusforsch. 1972;36(1):158-65. doi: 10.1007/BF01250306.

本文引用的文献

1

REVERSED PASSIVE HAEMAGGLUTINATION SYSTEMS FOR THE ESTIMATION OF TETANUS TOXINS AND ANTITOXINS.用于破伤风毒素和抗毒素测定的反向被动血凝系统

Immunology. 1965 Jan;8(1):74-80.

2

SIGNIFICANCE OF HAEMAGGLUTINATION-INHIBITING ANTIBODIES IN THE EVALUATION OF VACCINIAL REACTIONS.血凝抑制抗体在痘苗反应评估中的意义

Acta Microbiol Acad Sci Hung. 1964;11:139-45.

3

The reversed BDB technique. The agglutination of antibody-coated red blood cells by homologous antigen.反向BDB技术。同源抗原使抗体包被的红细胞发生凝集。

J Allergy. 1963 Mar-Apr;34:142-54. doi: 10.1016/0021-8707(63)90067-4.

4

Diphtheria antitoxin titration of human sera by haemagglutination.用血凝法测定人血清中的白喉抗毒素效价

Z Immun exp ther. 1962 Dec;124:401-10.

5

The use of spiral loops in serological and virological micro-methods.螺旋环在血清学和病毒学微量方法中的应用。

Acta Microbiol Acad Sci Hung. 1955;3(1-2):191-202.

6

A study of factors influencing the sensitivity to antibody of tanned, ovalbumin-coated, formalinized sheep red cells.一项关于影响鞣酸处理的、卵白蛋白包被的、甲醛固定的绵羊红细胞抗体敏感性因素的研究。

Immunology. 1967 Nov;13(5):459-68.

7

The significance of serological tests in controlling the success of smallpox revaccination.血清学检测在控制天花复种成功方面的意义。

Acta Microbiol Acad Sci Hung. 1966;13(2):97-112.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。