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用于估计抗牛痘抗体的反向被动血凝抑制法。

Reversed passive haemagglutination-inhibition method for the estimation of antivaccinia antibodies.

作者信息

Nyerges G

出版信息

Immunology. 1969 Sep;17(3):333-7.

PMID:5352004
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1455891/
Abstract

A reversed passive haemagglutination-inhibition (HAI) method has been evolved for measuring antivaccinia antibodies. Tanned sheep erythrocytes were sensitized with a bovine hyperimmune antivaccinia γ-globulin solution. The sensitized erythrocytes were agglutinated by a purified suspension of vaccinia virus. Haemagglutination was inhibited by sera containing antivaccinia antibodies and so the antivaccinia titres could be measured. Reversed passive HAI titres were compared with Hirst-type HAI, complement-fixing and neutralization titres in sera of persons vaccinated for the first time and also in persons revaccinated. A correlation was found between the reversed passive HAI titres and the neutralization titres.

摘要

已开发出一种反向被动血凝抑制（HAI）方法来检测抗牛痘抗体。用牛超免疫抗牛痘γ球蛋白溶液致敏鞣酸处理的绵羊红细胞。致敏红细胞被纯化的牛痘病毒悬液凝集。含抗牛痘抗体的血清可抑制血凝，因此可检测抗牛痘滴度。将首次接种疫苗者和再次接种疫苗者血清中的反向被动HAI滴度与赫斯特型HAI、补体结合和中和滴度进行比较。发现反向被动HAI滴度与中和滴度之间存在相关性。

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Immunology. 1969 Sep;17(3):333-7.
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引用本文的文献

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Vaccinia virus: kinetics of the hemagglutination-inhibition test and preparation of hemagglutinin.牛痘病毒：血凝抑制试验的动力学及血凝素的制备
Arch Gesamte Virusforsch. 1972;36(1):158-65. doi: 10.1007/BF01250306.

本文引用的文献

1
REVERSED PASSIVE HAEMAGGLUTINATION SYSTEMS FOR THE ESTIMATION OF TETANUS TOXINS AND ANTITOXINS.用于破伤风毒素和抗毒素测定的反向被动血凝系统
Immunology. 1965 Jan;8(1):74-80.
2
SIGNIFICANCE OF HAEMAGGLUTINATION-INHIBITING ANTIBODIES IN THE EVALUATION OF VACCINIAL REACTIONS.血凝抑制抗体在痘苗反应评估中的意义
Acta Microbiol Acad Sci Hung. 1964;11:139-45.
3
The reversed BDB technique. The agglutination of antibody-coated red blood cells by homologous antigen.反向BDB技术。同源抗原使抗体包被的红细胞发生凝集。
J Allergy. 1963 Mar-Apr;34:142-54. doi: 10.1016/0021-8707(63)90067-4.
4
Diphtheria antitoxin titration of human sera by haemagglutination.用血凝法测定人血清中的白喉抗毒素效价
Z Immun exp ther. 1962 Dec;124:401-10.
5
The use of spiral loops in serological and virological micro-methods.螺旋环在血清学和病毒学微量方法中的应用。
Acta Microbiol Acad Sci Hung. 1955;3(1-2):191-202.
6
A study of factors influencing the sensitivity to antibody of tanned, ovalbumin-coated, formalinized sheep red cells.一项关于影响鞣酸处理的、卵白蛋白包被的、甲醛固定的绵羊红细胞抗体敏感性因素的研究。
Immunology. 1967 Nov;13(5):459-68.
7
The significance of serological tests in controlling the success of smallpox revaccination.血清学检测在控制天花复种成功方面的意义。
Acta Microbiol Acad Sci Hung. 1966;13(2):97-112.

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