底物对肉碱乙酰转移酶旋光色散的影响。
The effects of substrates on the optical rotatory dipersion of carnitine acetyltransferase.
作者信息
Tipton K F, Chase J F
出版信息
Biochem J. 1969 Nov;115(3):517-21. doi: 10.1042/bj1150517.
Abstract
- The optical rotatory dispersion of carnitine acetyltransferase is altered in the presence of l-carnitine or acetyl-l-carnitine. These changes, which include an increase in the reduced mean residue rotation at 233nm. (M'), suggest that substrate binding causes the enzyme to unfold. 2. CoA and acetyl-CoA have no immediate effect on M' and CoA has no effect on the change in this parameter induced by l-carnitine. 3. The change in M' was used as a measure of the degree of saturation of the enzyme with carnitine substrates. Dissociation constants for the enzyme complexes with l-carnitine, d-carnitine and acetyl-l-carnitine were determined in this way. 4. Prolonged incubation of carnitine acetyltransferase in the presence of CoA leads to a small increase in the value of M' accompanied by irreversible inhibition of the enzyme. 5. Optical-rotatory-dispersion studies of two specifically inhibited enzyme forms are reported.
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本文引用的文献
1
Optical rotation and the conformation of polypeptides and proteins.多肽和蛋白质的旋光性与构象
Adv Protein Chem. 1961;16:401-544. doi: 10.1016/s0065-3233(08)60033-9.
2
Studies on the mechanism of enzyme-catalyzed oxidation reduction reactions. II. Methods for characterization of the mechanism for two-substrate systems.
Biochemistry. 1962 Jan;1:35-40. doi: 10.1021/bi00907a006.
3
Carnitine.肉碱
Vitam Horm. 1957;15:73-118. doi: 10.1016/s0083-6729(08)60508-7.
4
The substrate specificity of carnitine acetyltransferase.肉碱乙酰转移酶的底物特异性。
Biochem J. 1967 Aug;104(2):510-8. doi: 10.1042/bj1040510.
5
pH-dependence of carnitine acetyltransferase activity.肉碱乙酰转移酶活性的pH依赖性
Biochem J. 1967 Aug;104(2):503-9. doi: 10.1042/bj1040503.
6
Some kinetic studies on the mechanism of action of carnitine acetyltransferase.关于肉碱乙酰转移酶作用机制的一些动力学研究。
Biochem J. 1966 Apr;99(1):32-40. doi: 10.1042/bj0990032.
7
Conditions for the self-catalysed inactivation of carnitine acetyltransferase. A novel form of enzyme inhibition.肉碱乙酰转移酶自催化失活的条件。一种新型酶抑制形式。
Biochem J. 1969 Jan;111(2):225-35. doi: 10.1042/bj1110225.
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