Lane J W, Grrison R G, Field M F
J Bacteriol. 1969 Nov;100(2):1010-9. doi: 10.1128/jb.100.2.1010-1019.1969.
Fine details of the internal and external morphology of the yeastlike and mycelial phases of the dimorphic fungal pathogen Sporotrichum schenckii as seen in ultrathin sections are described and illustrated by electronphotomicrography. Comparisons of yeastlike phase ultrastructure were made using two different methods of fixation and embedding. The internal morphology of the two forms of yeastlike S. schenckii was in many ways similar to that of similarly dimorphic fungi and yeasts studied by other authors. However, the use of the glutaraldehyde-osmium in agar fixation technique suggests the presence of an electron transparent capsular or slime layer with associated electron dense microfibrils to be present external to the cell wall of the yeastlike phase but not the mycelial phase. Mycelial phase S. schenckii was found to contain many of the internal microstructures reported for other filamentous dimorphic fungi. Conidia production in agitated liquid culture was found to be restricted since only rare sessile conidia were observed.
通过电子显微镜照片描述并展示了在超薄切片中所见的双态真菌病原体申克孢子丝菌酵母样和菌丝体阶段的内部和外部形态的精细细节。使用两种不同的固定和包埋方法对酵母样阶段的超微结构进行了比较。两种形式的酵母样申克孢子丝菌的内部形态在许多方面与其他作者研究的类似双态真菌和酵母的形态相似。然而，在琼脂中使用戊二醛锇固定技术表明，在酵母样阶段的细胞壁外部存在一个电子透明的荚膜或黏液层以及相关的电子致密微纤维，而菌丝体阶段则没有。发现菌丝体阶段的申克孢子丝菌含有许多其他丝状双态真菌所报道的内部微观结构。由于仅观察到罕见的固着分生孢子，因此发现在搅拌液体培养中分生孢子的产生受到限制。
