高纯度免疫复合物中抗原的检测。

The examination of antigens in highly purified immune complexes.

作者信息

Heimer R, Abruzzo J L, Glick D

出版信息

Immunol Commun. 1979;8(5-6):545-53. doi: 10.3109/08820137909063253.

DOI:10.3109/08820137909063253

Abstract

A combination of gel filtration and affinity chromatography was used for preparation of immune complexes (IC) from sera of individuals with systemic lupus erythematosus (SLE) and others with no apparent disease. Overlaying of gels with patient or normal sera, counterstaining with 125I-protein A and autoradiography showed besides immunoglobulins, the presence of 6 unidentified antigens with molecular weights below 42,000 dalton. They were not exclusively associated with SLE IC.

摘要

采用凝胶过滤和亲和层析相结合的方法，从系统性红斑狼疮（SLE）患者及无明显疾病个体的血清中制备免疫复合物（IC）。用患者或正常血清覆盖凝胶，用¹²⁵I -蛋白A进行复染并放射自显影，结果显示，除免疫球蛋白外，还存在6种分子量低于42,000道尔顿的未鉴定抗原。它们并非仅与SLE免疫复合物相关。