Fretheim K, Iwai S, Feeney R E
Int J Pept Protein Res. 1979;14(5):451-6. doi: 10.1111/j.1399-3011.1979.tb01956.x.
The amino groups of ovomucoid, lysozyme and ovotransferrin have been extensively alkylated by reacting the proteins with various carbonyl reagents in the presence of sodim borohydride. The extent of modification ranged from 40 to 100%. Essentially monosubstitution was obtained with acetone, cyclopentanone, cyclohexanone and benzaldehyde, while 20--50% disubstitution was obtained with N-butanal and nearby 100% disubstitution was obtained with formaldehyde. Both the methylated and isopropylated derivatives of all three proteins were soluble and retained almost full biochemical activities, but introduction of the larger substituents caused precipitation with lysozyme and ovotransferrin.
通过在硼氢化钠存在下使蛋白质与各种羰基试剂反应，已对卵类粘蛋白、溶菌酶和转铁蛋白的氨基进行了广泛的烷基化。修饰程度在40%至100%之间。用丙酮、环戊酮、环己酮和苯甲醛基本上得到单取代，而用正丁醛得到20% - 50%的双取代，用甲醛得到近100%的双取代。所有三种蛋白质的甲基化和异丙基化衍生物均可溶，并几乎保留了全部生化活性，但引入较大的取代基会导致溶菌酶和转铁蛋白沉淀。
