Fitzpatrick F A, Stringfellow D A, Maclouf J, Rigaud M
J Chromatogr. 1979 Sep 14;177(1):51-60. doi: 10.1016/s0021-9673(00)92598-6.
Glass capillary gas chromatography of the prostaglandins was performed on a system including an all-glass, solventless injector; thermostable methylphenyl-polysiloxane glass capillary columns; and a conventional electron-capture detector fitted with a make-up gas tee. The principal stable metabolites of prostaglandin endoperoxide were separated as perfluorinated derivatives in 35 min. Detection limits equal or exceed those obtained for packed column separations and electron capture detection. Prostaglandin endoperoxide metabolic profiles from mammalian cell cultures were obtained using this system. These profiling studies are not possible with other chromatographic methods because of inferior resolution and sensitivity.
前列腺素的玻璃毛细管气相色谱分析是在一个包含全玻璃无溶剂进样器、热稳定的甲基苯基聚硅氧烷玻璃毛细管柱以及配有补充气三通的传统电子捕获检测器的系统上进行的。前列腺素内过氧化物的主要稳定代谢产物以全氟衍生物的形式在35分钟内分离出来。检测限等于或超过填充柱分离和电子捕获检测所获得的检测限。使用该系统获得了来自哺乳动物细胞培养物的前列腺素内过氧化物代谢谱。由于分辨率和灵敏度较差，其他色谱方法无法进行这些谱图研究。
