Lea A R, Kayaba W J, Hailey D M
J Chromatogr. 1979 Sep 14;177(1):61-8. doi: 10.1016/s0021-9673(00)92599-8.
A high-performance liquid chromatographic (HPLC) method is described which is capable of resolving cis- and trans-diethylstilbestrol (DES), DES mono- and dimethyl ethers and 4,4'-dihydroxystilbene. The mobile phase and internal standard used stabilise the cis-trans DES isomer ratio, and the method is capable of quantitating both isomers in dosage forms without derivatisation. Recovery of DES from tablets is quantitative. Results of tablet analyses using this method are compared with those obtained with the official spectrophotometric procedure.
本文描述了一种高效液相色谱（HPLC）方法，该方法能够分离顺式和反式己烯雌酚（DES）、DES单甲醚和二甲醚以及4,4'-二羟基芪。所使用的流动相和内标可稳定顺反式DES异构体比例，该方法无需衍生化即可定量测定剂型中的两种异构体。从片剂中回收DES是定量的。将使用该方法进行片剂分析的结果与采用官方分光光度法获得的结果进行了比较。
