反相高效液相色谱法测定葵花籽中的百草枯

Determination of paraquat in sunflower seeds by reversed-phase high-performance liquid chromatography.

作者信息

Paschal D C, Needham L L, Rollen Z J, Liddle J A

出版信息

J Chromatogr. 1979 Sep 14;177(1):85-90. doi: 10.1016/s0021-9673(00)92601-3.

Abstract

The herbicide paraquat was determined with extracts from 1-g samples of sunflower seeds. The liquid chromatography procedure utilized a microparticle (10 micron) C18 reversed-phase column and isocratic elution with 27% acetonitrile in water, 10 mM in the sodium salt of octanesulfonic acid. Eluted paraquat was detected at 254 and 280 nm and quantitated by paraquat internal standard peak height ratios. This procedure provided linear working curves over the concentration range of 0-20 microgram/g of paraquat. Recovery of paraquat varied from 89-101%, with an average recovery of 93%. Good agreement was obtained in the comparison of results of the described procedure with those from a well established UV procedure.

摘要

采用向日葵种子1克样品的提取物对除草剂百草枯进行了测定。液相色谱法采用微粒（10微米）C18反相柱，以含10 mM辛烷磺酸钠盐的27%乙腈水溶液进行等度洗脱。洗脱的百草枯在254和280纳米处进行检测，并通过百草枯内标峰高比进行定量。该方法在0 - 20微克/克百草枯的浓度范围内提供了线性工作曲线。百草枯的回收率在89% - 101%之间，平均回收率为93%。将所述方法的结果与成熟的紫外方法的结果进行比较，结果吻合良好。