Fok A K, Allen R D
J Protozool. 1979 Aug;26(3):463-70. doi: 10.1111/j.1550-7408.1979.tb04654.x.
To establish and grow Paramecium caudatum in mass axenic culture the culture medium of Soldo, Godoy & van Wagtendonk was modified by substituting phosphatidylethanolamine (PE) for TEM-4T and by a 10-fold increase in folic acid. Population densities of 4000 to 6000 cells/ml and a generation time of 20-26 h are regularly obtained. Optimal growth is obtained with PE-stigmasterol ratios between 40:1 to 400:1. Cells from 1-day-old axenic cultures have many lipid bodies aggregated in clumps (which disappear in 2 to 3 days) as well as foci of rough endoplasmic reticulum bordered by dictyosomes. The latter suggests a very active metabolism. Crystalline sheets found in both food vacuoles and lysosomes presumably play a role in digestion. Axenically grown cells also have abundant Golgi bodies (dictyosomes) and by late log phase become filled with lysosomes.
为了在大规模无菌培养中建立并培养尾草履虫，索尔托、戈多伊和范·瓦根东克的培养基进行了改良，用磷脂酰乙醇胺（PE）替代了TEM - 4T，并将叶酸浓度提高了10倍。通常可获得4000至6000个细胞/毫升的种群密度以及20 - 26小时的世代时间。当PE与豆甾醇的比例在40:1至400:1之间时可实现最佳生长。来自1日龄无菌培养物的细胞有许多脂质体聚集成团（2至3天内消失），还有由高尔基体界定的糙面内质网区域。后者表明代谢非常活跃。在食物泡和溶酶体中发现的晶体片可能在消化过程中起作用。无菌培养的细胞也有丰富的高尔基体（dictyosomes），到对数后期会充满溶酶体。
