Sakai K, Akima M, Nabata H
Jpn J Pharmacol. 1979 Apr;29(2):235-42. doi: 10.1254/jjp.29.235.
The isolated kidney of the recipient rat was perfused at a fixed flow rate with blood from a donor by a cross-circulation technique. The renal vasculature responded to the release of arterial occlusion with vasoconstriction, the magnitude of which was increased with increase in the duration of occlusion. ATP, ADP, AMP, adenosine, noradrenaline and 5-HT injected into the renal artery induced a prominent vasoconstriction; IMP and inosine had only a weak vasoconstrictor effect even in large doses. Theophylline reduced the vasoconstriction in response to arterial occlusion and to ATP and adenosine but did not affect that produced by noradrenaline or 5-HT. This suggests that adenine compounds, particularly adenosine, may play a role in the genesis of reactive vasoconstriction after arterial occlusion in the isolated rat kidney.
采用交叉循环技术，以固定流速用供体大鼠的血液灌注受体大鼠的离体肾脏。肾血管系统对解除动脉阻塞的反应是血管收缩，其收缩程度随阻塞时间的延长而增加。向肾动脉注射ATP、ADP、AMP、腺苷、去甲肾上腺素和5-羟色胺可引起明显的血管收缩；即使大剂量注射，肌苷酸和肌苷的血管收缩作用也很微弱。茶碱可减轻因动脉阻塞以及ATP和腺苷引起的血管收缩，但不影响去甲肾上腺素或5-羟色胺所引起的血管收缩。这表明腺嘌呤化合物，尤其是腺苷，可能在离体大鼠肾脏动脉阻塞后反应性血管收缩的发生过程中起作用。
