胆汁酸的微生物降解。简单节杆菌对胆酸的A环裂解及7α，12α-去羟基化作用。
Microbiological degradation of bile acids. Ring A cleavage and 7alpha, 12alpha-dehydroxylation of cholic acid by Arthrobacter simplex.
作者信息
Hayakawa S, Kanematsu Y, Fujiwara T
出版信息
Biochem J. 1969 Nov;115(2):249-56. doi: 10.1042/bj1150249.
Abstract
The metabolism of cholic acid by Arthrobacter simplex was investigated. This organism effected both ring a cleavage and elimination of the hydroxyl groups at C-7 and C-12 and gave a new metabolite, (4R)-4-[4alpha-(2-carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric acid, which was isolated and identified through its partial synthesis. A degradative pathway of cholic acid into this metabolite is tentatively proposed, and the possibility that the proposed pathway could be extended to the cholic acid degradation by other microorganisms besides A. simplex is discussed. The possibility that the observed reactions in vitro could occur during the metabolism of bile acids in vivo is considered.
摘要
研究了简单节杆菌对胆酸的代谢。该生物体既能实现A环裂解，又能消除C-7和C-12位的羟基，产生一种新的代谢产物，(4R)-4-[4α-(2-羧乙基)-3aα-六氢-7aβ-甲基-5-氧代茚满-1β-基]戊酸，通过部分合成对其进行了分离和鉴定。初步提出了胆酸降解为该代谢产物的途径，并讨论了除简单节杆菌外，该途径能否扩展到其他微生物对胆酸的降解。还考虑了体外观察到的反应在体内胆汁酸代谢过程中发生的可能性。
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