通过辅助细胞与结合在B细胞表面的免疫复合物相互作用诱导免疫应答。
Induction of an immune response through interaction of helper cells with an immune complex bound to the surface of B cells.
作者信息
Romano T J, Lerman S P, Bangasser S, Thorbecke G J, Nisonoff A
出版信息
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4555-8. doi: 10.1073/pnas.72.11.4555.
Adoptive anti-trinitrophenyl (Tnp) responses were elicited from Tnp-hemocyanin (Tnp-KLH)-primed cells by challenge with an immune complex of KLH and a Tnp conjugate of the Fab fragment of rabbit anti-KLH. Removal of T cells by treatment with anti-Thy 1.2 (phi C3H) + complement abolished this effect. Tnp-KLH primed cells that had been incubated with a Tnp conjugate of pneumococcal polysaccharide type III (Tnp-SIII), and which were then unable to respond to Tnp-KLH, made an anti-Tnp response upon incubation with rabbit anti-Dnp when transferred together with cells primed with rabbit gamma globulin. Since Tnp-KLH was not added in these experiments, it would appear that the cells were triggered by immune complexes on their surfaces consisting of Tnp-SIII and rabbit anti-Dnp with the help of T cells primed with rabbit gamma globulin; the presence of free antigen was evidently not required. Therefore, antigen-antibody complexes, when bound to the surface of B cells, may mediate T-B cell cooperation in the humoral immune response.
用钥孔戚血蓝蛋白（KLH）与兔抗KLH Fab片段的三硝基苯（Tnp）偶联物形成的免疫复合物刺激经Tnp-血蓝蛋白（Tnp-KLH）致敏的细胞，引发过继性抗三硝基苯（Tnp）反应。用抗Thy 1.2（ϕC3H）加补体处理去除T细胞可消除这种效应。用III型肺炎球菌多糖（Tnp-SIII）的Tnp偶联物孵育过的、因而无法对Tnp-KLH作出反应的Tnp-KLH致敏细胞，在与经兔γ球蛋白致敏的细胞一起转移并与兔抗二硝基苯（Dnp）孵育后，会产生抗Tnp反应。由于这些实验中未添加Tnp-KLH，似乎细胞是在经兔γ球蛋白致敏的T细胞的帮助下，被其表面由Tnp-SIII和兔抗Dnp组成的免疫复合物触发的；显然不需要游离抗原的存在。因此，抗原-抗体复合物与B细胞表面结合时，可能在体液免疫反应中介导T-B细胞协作。
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