Sato P S, Yoshida T O, Fukusima Y, Ono N, Yoshida K, Ito Y
Immunology. 1969 Dec;17(6):831-45.
Immune haemolysis was determined by rectilinear attenuance at 625 mμ ( = 1.3 mm, 1.25 mm), and the data obtained treated by von Krogh's empirical equation. The C′H/ml values obtained by the attenuance method had high correlation with those obtained by the conventional photometric method (541 mμ). Automatic complement titration was attempted, as a part of the basic studies on quantitative complement fixation tests.
通过在625毫微米(=1.3毫米,1.25毫米)处的直线衰减来测定免疫溶血,并将获得的数据用冯·克罗格经验方程进行处理。通过衰减法获得的C′H/毫升值与通过传统光度法(541毫微米)获得的值具有高度相关性。作为定量补体结合试验基础研究的一部分,尝试进行自动补体滴定。