乙型肝炎（表面）抗原固相放射免疫测定技术：逐步改进的效果

The solid phase radioimmunoassay technique for hepatitis B (surface) antigen: effects of progressive modification.

作者信息

Ni L Y, Dodd R Y, Brown N, Salmon R, Greenwalt T J

出版信息

Transfusion. 1975 Nov-Dec;15(6):608-11. doi: 10.1046/j.1537-2995.1975.15676082239.x.

DOI:10.1046/j.1537-2995.1975.15676082239.x

Abstract

Serum specimens submitted for confirmation of hepatitis B antigen (HBsAg) reactivity were tested by counterelectrophoresis (CEP) and by three modifications of the solid phase radioimmunoassay "sandwich" technique (SPRIA). In addition, the specificity of each reaction was determined for two of the SPRIA test methods. One hundred seventy-five of the 688 specimens were reactive by all techniques and a further 60 ere specifically reactive by two of modifications of SPRIA. An additional five specimens were positive by a third SPRIA modification. The primary SPRIA technique, using a homologous guinea pig antibody sandwich, detected 184 nonspecific reactive samples. With the same test done at 45C with a reduced incubation time, there were only 37 nonspecific reactive samples. None of the nonspecific samples was detected when the SPRIA technique used a heterologous guinea pig-human antibody sandwich. The results provide a basis for estimating the effectiveness of the SPRIA technique for HBsAg testing.

摘要

提交用于确认乙型肝炎抗原（HBsAg）反应性的血清标本，通过对流免疫电泳（CEP）以及固相放射免疫分析“夹心”技术（SPRIA）的三种改良方法进行检测。此外，还针对两种SPRIA检测方法确定了每种反应的特异性。688份标本中有175份通过所有技术检测呈反应性，另有60份通过两种SPRIA改良方法特异性呈反应性。还有5份标本通过第三种SPRIA改良方法呈阳性。使用同源豚鼠抗体夹心的原始SPRIA技术检测到184份非特异性反应性样本。在45℃下进行相同测试并缩短孵育时间后，非特异性反应性样本仅37份。当SPRIA技术使用异源豚鼠 - 人抗体夹心时，未检测到任何非特异性样本。这些结果为评估SPRIA技术用于HBsAg检测的有效性提供了依据。