Tembhare D B, Thakare V K
Z Mikrosk Anat Forsch. 1975;89(1):108-27.
The ovaries consist of large number of panoistic ovarioles in the last instar nymph and the adult dragonfly Orthetrum chrysis (Selys). In the nymph the vitellaria are compactly filled with the primary oocytes and the vitellogenesis takes place only in the adult stage. During vitellogenesis oocytes change widely in their shape, size and cytological organisation and their developmental stages can be divided into pre-vitellogenic, early-vitellogenic, vitellogenic, late-vitellogenic and maturation age. PAS-positive material appears first around the germinal vesicle in the early-vitellogenic stage and lateron it migrates towards the periphery. Glycogen appears in the late-vitellogenic stage. DNA is abundantly present in the nuclei of the oocytes during the pre-vitellogenic and completely absent in early-vitellogenic, vitellogenic, late-vitellogenic and maturation stages. It is observed in the nuclei of follicular epithelial cells of all the stages. RNA is abundantly present in cytoplasm of the pre-vitellogenic oocytes but lateron is gradually decreases. During the early-vitellogenic and vitellogenic stages high concentration of RNA in the follicular epithelial cells has been observed. The protein bodies appear first in the interfollicular spaces and towards the periphery of the oocytes just near the enveloping follicular epithelial cells, during the early-vitellogenic stage suggesting the formation of yolk proteins from the haemolymph. In Orthetrum chrysis the sudanophilic bodies appear first in the follicular cells and then lie in the peripheral region of the oocytes suggesting the incorporation of yolk lipid either from the follicular epithelium or from the haemolymph through the follicular epithelium. The phospholipids are synthesised in pre-vitellogenic to the late-vitellogenic stages. In the late-vitellogenic stages the phospholipid granules are present abundantly in the follicular epithelium while in the maturation stage they disappear suggesting their utilisation in the formation of membranes like vitelline and chorion. The neutral fats are present in the form of large number of droplets in the oocytes during the maturation stage.
在最后一龄若虫和成年蜻蜓黄蜻（Orthetrum chrysis，赛氏）中，卵巢由大量无滋式卵巢小管组成。在若虫阶段，卵黄腺紧密充满初级卵母细胞，卵黄发生仅在成虫阶段进行。在卵黄发生过程中，卵母细胞在形状、大小和细胞学组织上发生广泛变化，其发育阶段可分为卵黄发生前期、卵黄发生早期、卵黄发生期、卵黄发生后期和成熟期。PAS阳性物质在卵黄发生早期首先出现在生发泡周围，随后向周边迁移。糖原出现在卵黄发生后期。DNA在卵黄发生前期大量存在于卵母细胞核中，在卵黄发生早期、卵黄发生期、卵黄发生后期和成熟期完全不存在。在所有阶段的滤泡上皮细胞核中均观察到DNA。RNA在卵黄发生前期的卵母细胞细胞质中大量存在，但随后逐渐减少。在卵黄发生早期和卵黄发生期，观察到滤泡上皮细胞中RNA浓度较高。蛋白质体在卵黄发生早期首先出现在滤泡间空间，并朝向卵母细胞周边，靠近包围的滤泡上皮细胞，这表明卵黄蛋白是由血淋巴形成的。在黄蜻中，嗜苏丹体首先出现在滤泡细胞中，然后位于卵母细胞的周边区域，这表明卵黄脂要么来自滤泡上皮，要么通过滤泡上皮来自血淋巴。磷脂在卵黄发生前期到卵黄发生后期合成。在卵黄发生后期，磷脂颗粒大量存在于滤泡上皮中，而在成熟期它们消失，表明它们被用于形成卵黄膜和卵壳等膜结构。中性脂肪在成熟期以大量液滴的形式存在于卵母细胞中。
