Deshpande D J, Srivastava K P
Z Mikrosk Anat Forsch. 1981;95(6):1035-47.
A histological and histochemical study of the ovary of Dysdercus koenigii has been carried out to ascertain the origin of protein, lipid and carbohydrate components of the yolk during vitellogenesis. Germaria of the telotrophic ovarioles of this insect comprise mononucleate and binucleate trophocytes, prefollicular cells and oogonia ensheathed in two membranes. The follicular epithelium derived from the prefollicular cells is multilayered in the beginning of the oocyte development but is progressively reduced to one layer in later stages. Chromatin material in the binucleate trophocytes condenses to form DNA-positive blobs. RNA-positive material flowing from the nutritive cords can be seen at the nutritive cord-oocyte junction. Protein bodies appear first in the intercellular spaces of follicular epithelium during the early vitellogenic stages indicating the supply of yolk protein from the haemolymph. Lipids originate as L1 bodies made up of unsaturated phospholipids in the nucleus (germinal vesicle). These bodies by addition of saturated triglycerides from haemolymph change to L2 bodies, which in turn, knock off phospholipid part and change of L3 bodies. Polysaccharides come as extrusions from the germinal vesicle while glycogen is first seen in the intercellular spaces of follicular epithelium and then passes into the oocyte indicating its origin in the haemolymph.
对红蝽卵巢进行了组织学和组织化学研究，以确定卵黄生成过程中卵黄的蛋白质、脂质和碳水化合物成分的来源。这种昆虫的端滋式卵巢小管的生殖腺由单核和双核滋养细胞、滤泡前细胞和被两层膜包裹的卵原细胞组成。源自滤泡前细胞的滤泡上皮在卵母细胞发育开始时是多层的，但在后期逐渐减少到一层。双核滋养细胞中的染色质物质浓缩形成DNA阳性斑点。在营养索-卵母细胞交界处可以看到从营养索流出的RNA阳性物质。在卵黄生成早期，蛋白质体首先出现在滤泡上皮的细胞间隙中，这表明血淋巴提供了卵黄蛋白。脂质起源于细胞核（胚泡）中由不饱和磷脂组成的L1体。这些体通过添加来自血淋巴的饱和甘油三酯转变为L2体，L2体又去掉磷脂部分转变为L3体。多糖从胚泡中挤出，而糖原首先出现在滤泡上皮的细胞间隙中，然后进入卵母细胞，表明其起源于血淋巴。
