Schmidt C
Am Ind Hyg Assoc J. 1979 Dec;40(12):1085-90. doi: 10.1080/15298667991430730.
This paper describes a one-tube sample preparation method for blood lead determination which is relatively simple to perform and which does not require background correction for matrix effects. The red cells are lysed with a dilute potassium cyanide solution and heat. A citrate buffer is added, and the solution is then extracted with ammonium pyrrolidine dithiocarbamate and methyl isobutyl ketone. An aliquot of the MIBK layer is applied to a carbon rod atomizer and the peak at 217.0 nm is measured. The method demonstrates an overall in-run reproducibility of 1.39 microgram/100 mL blood standard deviation, a between-run standard deviation of 2.27 microgram/100 mL blood and a sensitivity of 0.012 microgram/mL/1% nm.
本文描述了一种用于血铅测定的单管样品制备方法，该方法操作相对简单，且无需对基质效应进行背景校正。用稀氰化钾溶液和加热使红细胞裂解。加入柠檬酸盐缓冲液，然后用吡咯烷二硫代氨基甲酸铵和甲基异丁基酮萃取该溶液。取一份甲基异丁基酮层溶液注入碳棒原子化器，测量217.0nm处的峰。该方法的总体批内重现性为1.39微克/100毫升血液标准差，批间标准差为2.27微克/100毫升血液，灵敏度为0.012微克/毫升/1%纳米。
